Tender For purchase of chemicals - purchase of chemicals, 2,4-d size 250g2,4-d is a synthetic auxin widely used in plant tissue culture to promote cell division and callus formation. it plays a key role in inducing dedifferentiation of plant cells, making it essential for callus induction and somatic embryogenesis. it is commonly included in culture media at specific concentrations to support plant regeneration and transformation experiments., agar powdersize 1kgagar powder is a polysaccharide derived from red algae, widely used as a solidifying agent in microbiological and plant tissue culture media. it forms a stable gel when cooled, providing a firm surface for the growth of microorganisms and plant cells. agar is chemically inert and does not interfere with most biological processes, making it ideal for culturing and experimental applications., casein enzyme size 500gcasein enzyme refers to proteolytic enzymes that hydrolyze casein, a milk protein, into smaller peptides and amino acids. in microbiology and molecular biology, these enzymes are used to study protein digestion and are components of media (as casein hydrolysate) to provide a rich nitrogen source for microbial growth. they are also used in enzymatic assays to evaluate protease activity., ms mediasize 25lms medium is a widely used plant tissue culture medium formulated by murashige and skoog, containing essential macro- and micronutrients, vitamins, and a carbon source (usually sucrose) to support plant cell growth and development. it provides an optimal nutrient balance for processes such as seed germination, callus induction, and plant regeneration, and can be supplemented with plant growth regulators like auxins and cytokinins depending on the experimental requirement., bap size 5gbap is a synthetic cytokinin used in plant tissue culture to promote cell division and shoot formation. it plays a key role in stimulating axillary bud growth and shoot proliferation, making it essential for plant regeneration and micropropagation. it is commonly added to culture media in specific concentrations depending on the plant species and experimental requirements., sodium hypochlorite size 5lsodium hypochlorite is a strong oxidizing agent commonly used as a disinfectant and sterilizing agent in laboratory protocols. in plant tissue culture and dna extraction procedures, it is used for surface sterilization of plant materials to eliminate microbial contaminants. it is typically used in diluted form and followed by thorough rinsing with sterile water to remove residualchemicals., timentinsize 2gtimentin is a combination antibiotic containing ticarcillin and clavulanic acid, commonly used in plant transformation experiments. it is added to culture media to eliminate agrobacterium tumefaciens after co-cultivation while allowing plant tissues to grow. clavulanic acid inhibits -lactamase enzymes, enhancing the effectiveness of ticarcillin against bacterial cells., kanamycin size 5gkanamycin is an aminoglycoside antibiotic used in molecular biology to select bacteria or plant cells carrying the kanamycin resistance (nptii) gene. it inhibits protein synthesis by binding to the 30s ribosomal subunit, leading to bacterial cell death. it is commonly added to culture media to maintain selective pressure during cloning and plant transformation experiments., rifampicin size 5grifampicin is an antibiotic used in molecular biology to select and maintain agrobacterium tumefaciens strains carrying rifampicin resistance. it inhibits bacterial rna synthesis by binding to dna-dependent rna polymerase, preventing transcription. it is commonly added to culture media to suppress unwanted bacterial growth and ensure strain stability during plant transformation experiments., edta size 500gedta is a chelating agent used in molecular biology to bind divalent metal ions such as mg and ca . by removing these ions, it inhibits nuclease activity and protects dna from degradation during extraction and storage. it is
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Tender For purchase of chemicals - purchase of chemicals, diluent for dna extractionsize 500mla diluent used in dna extraction is typically te buffer (10 mm tris-hcl, 1 mm edta, ph ~8.0) or nuclease-free water, used to dissolve and dilute extracted dna. tris maintains a stable ph, while edta chelates divalent cations (e.g., mg ), thereby inhibiting dnase activity and protecting dna from degradation. the diluent must be sterile and free of nucleases to ensure the integrity of dna for downstream applications such as pcr, sequencing, and cloning., luria bertani mediumsize 500gluria bertani medium is a nutrient-rich growth medium commonly used for culturing bacteria such as escherichia coli and agrobacterium tumefaciens. it contains tryptone (10 g/l), yeast extract (5 g/l), and sodium chloride (10 g/l), which provide essential nutrients, vitamins, and osmotic balance for bacterial growth. lb medium supports rapid cell growth and is widely used in molecular biology for cloning, plasmid propagation, and transformation experiments. for solid media, agar (15g/l) is added., chloroform (high analytical reagent grade) size 2.5lchloroform (high analytical reagent grade) is a high-purity organic solvent used in dna extraction, particularly in phenol chloroform methods, to separate proteins and other contaminants from nucleic acids. it enhances phase separation by forming a distinct organic layer, allowing dna to remain in the aqueous phase. due to its toxicity and volatility, it must be handled under a fume hood with proper safety precautions., depcsize 25mldepc is a chemical used to inactivate rnases in water and laboratory solutions, making them suitable for rna work. it modifies histidine residues in proteins, thereby deactivating rnase enzymes that can degrade rna. depc-treated solutions are typically incubated and then autoclaved to remove residual depc, ensuring they are safe for use in downstream applications such as rna extraction and cdna synthesis., tb green master mixsize 200rnxtb green master mix is a ready-to-use reagent used in real-time pcr (qpcr) for dna amplification and detection. it contains tb green dye (a double-stranded dna-binding fluorescent dye), dna polymerase, dntps, mg , and reaction buffer. the dye binds to amplified double-stranded dna and emits fluorescence, allowing real-time monitoring of pcr amplification. it is commonly used for gene expression analysis and quantification of dna., dna loading dyesize 10 inchdna loading dye is a solution added to dna samples before agarose gel electrophoresis. it contains tracking dyes (such as bromophenol blue and xylene cyanol) that allow monitoring of dna migration during the run, and a dense agent (such as glycerol or sucrose) that helps the sample sink into the wells. it may also include edta to protect dna from nuclease degradation., metaloopa metal loop is a reusable laboratory tool made of platinum or nichrome wire, used for transferring and inoculating microorganisms onto culture media. it is sterilized by heating in a flame until red hot before and after use to prevent contamination. it is commonly used in microbiology for streaking plates and handling bacterial cultures.